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Katsumasa FUJITA

Professor, Ph. D.
Department of Applied Physics, Osaka University
2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
office: P2-300, Suita Campus, Osaka University
phone: +81-6-6879-7847

Symposium: BRI 2019

We organized a symposium: Biomedical Raman Imaging 2019 on 24 - 26 Nove, 2019 in Osaka. There were 129 participants from many different countries!

A report in Spectroscopy, 35 (4), 43–46 (2020).

Research Topics

HeLa cell stained with AttoR6G phalloidin

Super-resolution fluorescence microscopy : SAX microscopy
We are developing optical microscopy techniques which can resolve fine structures of samples with the spatial resolution beyond the diffraction limit of light. By using saturated excitation (SAX) of fluorescence molecules, we induce nonlinear fluorescence emission that contribute to produce super-resolution images. The SAX miroscope can be realized by simply modulating laser intensity and demodulating fluorescence signal in a typical confocal microscope.

The figure shows the fluorescence images of a HeLa cell stained with AttoR6G phalloidin (xz cross-section). SAX microscopy can image fine structures that can not be resolved by conventional confocal microscopy.

Nawa et al., APL Photonics (2018).
Oketani et al., Opt. Lett. (2017).
Yonemaru et al., Phys. Rev. Applied (2015).
Chu et al., Phys. Rev. Lett. (2014).
Yamanaka et al., J. Biomed. Opt. (2013).
Fujita et al., Phys. Rev. Lett. (2007)

753 cm-1 : Cytochrome
1686 cm-1 : Protein
2852 cm-1 : Lipid

Movie by Raman
Cell division: cytokinesis
Cell division: mitosis
Raman microscopy for imaging of biological samples
We are developing Raman microscopes to image and analyze biomolecules in living cells. Raman scattering spectra show molecular vibrations in a sample, that contain rich information about species, conditions and environments of molecules. We have developed a slit-scanning Raman microscope and applied it to imaging of biomolecule dynamics in a living cell. We are also developing a higher-sensitive Raman detection technique for cellular imaging by using surface-enhanced Raman scattering and Raman tags to image small molecules.

The figure shows a Raman image of living HeLa cells. Molecular vibrations detected by Raman spectra produce the distribution of proteins and lipids in the cells.

Bando et al., Biomed. Opt. Express. (2022).
SPIE Newroom (2016).
Wanatabe et al., Nat. Commun. (2015).
Palonpon et al, Nat. Protoc. (2013).
Okada et al., PNAS (2012).
Ando et al., Nano Lett. (2011).
Hamada et al., J. Biomed. Opt. (2008).

Alkyne (EdU), Cytochrome , Lipid

Raman tag imaging
Raman tag imaging is a new technique to observe small molecules in living cells and tissues. Small molecules are too "small" to be labeled by fluorescent dyes and could not be observed in the physiological conditions. We have demonstrated the use of Raman tag to visualize small molecules. Raman tag, such as an alkyne, shows a Raman band district from intracellular molecules and allows us to observe small molecules labeled by Raman tags with Raman microscopy. The Raman tag also realizes multiplexed imaging of many different molecules via the extremely narrow emission band of Raman scattering. We are also working on the application of the Raman tag technique for pharmaceutical researches.

Dodo et al, JACS (2022). Koike et al, ACS Nano (2020).
Ando et al, JACS (2016).
Ando et al, PNAS (2015).
Yamakoshi et al, Bioorg. Med. Chem. Lett. (2015).
Yamakoshi et al, Chem Commun. (2014).
Palonpon et al, Nat. Protoc. (2013).
Yamakoshi et al., JACS (2011).

Original Papers

  • M. Li, Y. Nawa, S. Ishida, Y. Kanda, S. Fujita, and K. Fujita, "Label-free chemical imaging of cytochrome P450 activity by Raman microscopy," Commun Biol., 5, 778 (2022)
  • M. Li, H.-X. Liao, K. Bando, Y. Nawa, S. Fujita, and K. Fujita, "Label-free monitoring of drug-induced cytotoxicity and its molecular fingerprint by live-cell Raman and autofluorescence imaging," Anal. Chem., 94 (28) 10019–10026 (2022).
  • A. Nakayama, Y. Kumamoto M. Minoshima, K. Kikuchi, A. Taguchi, and K. Fujita, "Photoinitiator-free two-photon polymerization of biocompatible materials for 3D micro/nanofabrication,”Adv. Opt. Mater., 2200474 (2022).
  • K. Bando, S. Yabuuchi, M. Li, T. Kubo, R. Oketani, N. I. Smith, and K. Fujita, "Bessel-beam illumination Raman microscopy,” Biomed. Opt. Express. 13 (6) 3161-3170 (2022).
  • K. Temma, R. Oketani, R. Lachmann, T. Kubo, N. I. Smith, R. Heintzmann, and K. Fujita, "Saturated-excitation image scanning microscopy," Opt. Express. 30 (8) 13825-13838 (2022).
  • K. Koike, N. I. Smith, and K. Fujita, “Spectral focusing in picosecond pulsed stimulated Raman scattering microscopy,” Biomed. Opt. Express., 13 (2), 995-1004 (2022).
  • H. Lee, H. Yoo, G. Moon, K.-A. Toh, K. Mochizuki, K. Fujita, and D. Kim, "Super-resolved Raman microscopy using random structured light illumination: concept and feasibility," J. Chem. Phys. 155, 144202 (2021).
  • T. Kubo, K. Temma, K. Sugiura, H. Shinoda, K. Lu, N. I. Smith, T. Matsuda, T. Nagai, K. Fujita, "Visible-wavelength multiphoton activation confocal microscopy," ACS Photonics, 8 (9) 2666–2673 (2021).
  • T. Ichimura, T. Kakizuka, K. Horikawa, K. Seiriki, A. Kasai, H. Hashimoto, K. Fujita, T. M. Watanabe, T. Nagai, “Exploring rare cellular activity in more than one million cells by a trans-scale scope,” Sci. Rep., 11, 16539 (2021).
  • H. Kawagoe, J. Ando, M. Asanuma, K. Dodo, T. Miyano, H. Ueda, M. Sodeoka, K. Fujita, “Multiwell Raman plate reader for high-throughput biochemical screening,” Sci. Rep., 11, 15742 (2021).
  • J. Ando, H. Kawagoe, A. Nakamura, R. Iino and K. Fujita, "Label-free monitoring of crystalline chitin hydrolysis by chitinase based on Raman spectroscopy," Analyst, 146, 4087-4094 (2021).
  • K. Nishida, H. Sato, R. Oketani, K. Mochizuki, K. Temma, Y. Kumamoto, H. Tanaka, K. Fujita, "Using saturated absorption for super‐resolution laser scanning transmission microscopy," J. Microsc. (online).
  • F. Tai, K. Koike, H. Kawagoe, J. Ando, Y. Kumamoto, N. I. Smith, M. Sodeoka, K. Fujita, "Detecting nitrile-containing small molecules by infrared photothermal microscopy," Analyst, 146, 2307-2312 (2021).
  • T. Kubo, K. Temma, N. I. Smith, K. Lu, T. Matsuda, T. Nagai, and K. Fujita, "Hyperspectral two-photon excitation microscopy using visible wavelength," Opt. Lett., 46 (1) 37–40 (2021).
  • S. A. Hussain, T. Kubo, N. Hall, D. Gala, K. Hampson, R. Parton, M. A. Phillips, M. Wincott, K. Fujita, I. Davis, I. Dobbie, M. J. Booth, "Wavefront‐sensorless adaptive optics with a laser‐free spinning disk confocal microscope," J. Microsc. (Online).
  • H. Lee, K. Kang, K. Mochizuki, C. Lee, K.-A. Toh, S. Lee, K. Fujita, D. Kim, "Surface plasmon localization-based super-resolved Raman microscopy," Nano Lett., 20 (12) 8951–8958 (2020).
  • K. Koike, K. Bando, J. Ando, H. Yamakoshi, N. Terayama, K. Dodo, N. I. Smith, M. Sodeoka, K. Fujita, "Quantitative drug dynamics visualized by alkyne-tagged plasmonic-enhanced Raman microscopy," ACS NANO, 14(11), 15032–15041 (2020).
  • A. Taguchi, A. Nakayama, R. Oketani, S. Kawata, K. Fujita, “Multiphoton-excited deep ultraviolet photolithography for 3D nanofabrication,” ACS Appl. Nano Mater., 3(11), 11434–11441 (2020).
  • Y.-S. Duh, Y. Nagasaki, Y.-L. Tang, P.-H. Wu, H.-Y. Cheng, T.-H. Yen, H.-X. Ding, K. Nishida, I. Hotta, J.-H. Yang, Y.-P. Lo, K.-P. Chen, K. Fujita, C.-W. Chang, K.-H. Lin, J. Takahara, and S.-W. Chu, "Giant photothermal nonlinearity in a single silicon nanostructure." Nat Commun 11, 4101 (2020).
  • K. Nishida, G. Deka, N. I. Smith, S.-W. Chu*, K. Fujita*, "Nonlinear scattering of near-infrared light for imaging plasmonic nanoparticles in deep tissue," ACS Photonics, 7, 2139–2146 (2020).
  • K. Bando, Z. Zhang, D. Graham, K. Faulds, K. Fujita*, S. Kawata, "Dynamic pH measurement of intracellular pathways using nano-plasmonic assemblies," Analyst, 145, 5768 - 5775 (2020).
  • M. Tanuma, A. Kasai, K. Bando, N. Kotoku, K. Harada, M. Minoshima, K. Higashino, A. Kimishima, M. Arai, Y. Ago, K. Seiriki, K. Kikuchi, S. Kawata, K. Fujita. and H. Hashimoto, “Direct visualization of an antidepressant analog using surface-enhanced Raman scattering in the brain,” JCI Insight, 5(6), e133348 (2020).
  • R. Oketani, H. Suda, K. Uegaki, T. Kubo, T. Matsud, M. Yamanaka, Y. Arai, N. I. Smith, T. Nagai, K. Fujita, "Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging," J. Biomed. Opt., 25(1), 014502 (2020).
  • Y. Nagasaki, T. Kohno, K. Bando, H. Takase, K. Fujita, J. Takahara, "Adaptive camouflage using VO2 optical antennas with subwavelength resolution," J. Phys. Chem. B, 123 (20) 4358-4372 (2019).
  • Y. Kumamoto, K. Mochizuki, K. Hashimoto, Y.Harada, H. Tanaka, K. Fujita*, "High-Throughput cell imaging and classification by narrowband and low-spectral-resolution Raman microscopy," J. Phys. Chem. B., 123 (12), 2654 (2019).
  • T. Morimoto, L.-d. Chiu*, H. Kanda, H. Kawagoe, T. Ozawa, M. Nakamura, K. Nishida, K. Fujita*, T. Fujikado, "Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction," Analyst, 144, 2540 (2019).
  • Z. Zhang*, K. Bando, K. Mochizuki, A. Taguchi, K. Fujita*, and S. Kawata, "Quantitative evaluation of SERS nanoparticles for intracellular pH sensing at a single particle level," Anal. Chem., 91 (5), 3254 (2019).
  • Y. Nawa, Y. Yonemaru, A. Kasai, R. Oketani, H. Hashimoto, N. I. Smith, and K. Fujita*, "Saturated excitation microscopy using differential excitation for efficient detection of nonlinear fluorescence signals," APL Photonics 3, 080805 (2018).
  • A. Germond, T. Ichimura, L.-d. Chiu, K. Fujita, T. M. Watanabe, H. Fujita, "Cell type discrimination based on image features of molecular component distribution," Sci Rep., 8, 11726 (2018).
  • G. Deka, K. Nishida, K. Mochizuki, H.-X. Ding, K. Fujita*, and S.-W. Chu*, “Resolution enhancement in deep-tissue nanoparticle imaging based on plasmonic saturated excitation microscopy,” APL Photonics 3, 031301 (2018).
  • A. Doi, R. Oketani, Y. Nawa, and K. Fujita, “High-resolution imaging in two-photon excitation microscopy using in situ estimations of the point spread function”,Biomed. Opt. Express 9, 202 (2018).
  • Z. Zhang*, K. Bando, A. Taguchi, K. Mochizuki, K. Sato, H. Yasuda, K. Fujita*, and S. Kawata, “Au-Protected Ag Core/Satellite Nanoassemblies for Excellent Extra-/Intracellular Surface-Enhanced Raman Scattering Activity”, ACS Appl. Mater. Interfaces, 9 (50), 44027 (2017).
  • K. Seiriki, A. Kasai, T. Hashimoto, W. Schulze, M. Niu, S. Yamaguchi, T. Nakazawa, K. Inoue, S. Uezono, M. Takada, Y. Naka, H. Igarashi, M. Tanuma, J. A. Waschek, Y. Ago, K. F. Tanaka, A. Hayata-Takano, K. Nagayasu, N. Shintani, R. Hashimoto, Y. Kunii, M. Hino, J. Matsumoto, H. Yabe, T. Nagai, K. Fujita, T. Matsuda, K. Takuma, A. Baba, H. Hashimoto, "High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates," Neuron 94, 1085 (2017).
  • L.-d. Chiu, T. Ichimura, T. Sekiya, H. Machiyama, T. Watanabe, H. Fujita, T. Ozawa, K. Fujita*, "Protein expression guided chemical profiling of living cells by hybrid fluorescence-Raman microscopy," Sci. Rep. 7: 43569 (2017).
  • R. Oketani, A. Doi, N. I. Smith, Y. Nawa, S. Kawata, and K. Fujita*, "Saturated two-photon excitation fluorescence microscopy with core-ring illumination," Opt. Lett., 42 (3) 571 (2017).
  • T. Ichimura , L.-d. Chiu , K. Fujita , H. Machiyama , T. Yamaguchi , T. Watanabe, H. Fujita, "Non-label immune cell state prediction using Raman spectroscopy," Sci. Rep. 6: 37562 (2016).
  • J. Ando, M. Asanuma, K. Dodo, H. Yamakoshi, S. Kawata, K. Fujita* and M. Sodeoka*, "Alkyne-tag SERS screening and identification of small-molecule-binding sites in protein," J. Am. Chem. Soc., 38 (42) 13901 (2016).
  • Y.-T. Chen, P.-H. Lee, P.-T. Shen, J. Launer, R. Oketani, K.-Y. Li, Y.-T. Huang, K. Masui, S. Shoji, K. Fujita, S.-W. Chu, “Study of nonlinear plasmonic scattering in metallic nanoparticles,” ACS Photonics, 3 (8), 1432–1439 (2016).
  • H.-Y. Wu, Y.-T. Huang, P.-T. Shen, H. Lee, R. Oketani, Y. Yonemaru, M. Yamanaka, S. Shoji, S. Kawata, C.-W. Chang, K.-H. Lin, K. Fujita, S.-W. Chu, "Ultrasmall all-optical plasmonic switch and its application to superresolution imaging," Sci. Rep., 6: 24293 (2016).
  • H. Lee, K.-Y. Li, Y.-T. Huang, P.-T. Shen, G. Deka, R. Oketani, Y. Yonemaru, M. Yamanaka, K. Fujita, S.-W. Chu, "Measurement of scattering nonlinearities from a single plasmonic nanoparticle," J. Vis. Exp. (107), e53338 (2016).
  • Y. Kumamoto, K. Fujita, N. I. Smith, and S. Kawata, "Deep-UV biological imaging by lanthanide ion molecular protection," Biomed. Opt. Express, 7 (1), 158-170 (2016).
  • K. Watanabe, A. F. Palonpon, N. I. Smith, L.-d. Chiu, A. Kasai, H. Hashimoto, S. Kawata, K. Fujita, "Structured line illumination Raman microscopy," Nat. Commun. 6:10095 (2015).
  • K. Bando, N. I. Smith, K. Fujita, S. Kawata, "Analysis of dynamic SERS spectra measured with a nanoparticle during intracellular transportation in 3D," J. Opt., 17, 114023 (2015).
  • M. Yamanaka, K. Saito, N. I. Smith, Y. Arai, K. Uegaki, Y. Yonemaru, K. Mochizuki, S. Kawata, T. Nagai, K. Fujita, "Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging," J. Biomed. Opt., 20 (10), 101202 (2015).
  • A. Hashimoto, Y. Yamaguchi, L.-d. Chiu, C. Morimoto, K. Fujita, M. Takedachi, S. Kawata, S. Murakami, E. Tamiya, "Time-lapse Raman imaging of osteoblast differentiation," Sci. Rep., 5, 12529 (2015).
  • Y. Yonemaru, A. F. Palonpon, S. Kawano, N. I. Smith, S. Kawata, and K. Fujita, "Super-spatial- and -spectral-resolution in vibrational imaging via saturated coherent anti-Stokes Raman scattering," Phys. Rev. Applied, 4, 014010 (2015).
  • Z. Zheng, S. Mizukami, K. Fujita, and K. Kikuchi, "An enzyme-responsive metal-enhanced near-infrared fluorescence sensor based on functionalized gold nanoparticles," Chem. Sci., 6, 4934-4939 (2015).
  • T. Ichimura, L.-d. Chiu, K. Fujita, H. Machiyama, S. Kawata, T. M. Watanabe, H. Fujita, "Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging," Sci. Rep., 5, 11358 (2015).
  • D. K. Tiwari, Y. Arai, M. Yamanaka, T. Matsuda, M. Agetsuma, M. Nakano, K. Fujita, T. Nagai, "Fast positively photoswitchable fluorescent protein for ultra-low laser power RESOLFT nanoscopy," Nat. Methods, 12, 515-518 (2015).
  • J. Ando, M. Kinoshita, J. Cui, H. Yamakoshi, K. Dodo, K. Fujita, M. Murata, M. Sodeoka, "Sphingomyelin distribution in lipid rafts of artificial monolayer membranes visualized by Raman microscopy," Proc. Natl. Acad. Sci., 112 (15), 4558-4563 (2015).
  • K. Mochizuki, L. Shi, S. Mizukami, M. Yamanaka, M. Tanabe, W.-T. Gong, A. F. Palonpon, S. Kawano, S. Kawata, K. Kikuchi, K. Fujita, "Nonlinear fluorescence imaging by photoinduced charge separation," Jpn. J. Appl. Phys., 54, 042403 (2015).
  • A. J. Hobro, N. Pavillon, K. Fujita, M. Ozkan, C. Coban, N. I. Smith, "Label-free Raman imaging of the macrophage response to the malaria pigment hemozoin," Analyst, 140, 2350-2359 (2015).
  • Y. Saito and K. Fujita, "Direct electron density modulation of surface plasmons with a scanning electron microscope," Appl. Phys. Express 8, 015001 (2015).
  • H. Yamakoshi, A. Palonpona, K. Dodo, J. Ando, S. Kawata, K. Fujita, M. Sodeoka, "A sensitive and specific Raman probe based on bisarylbutadiyne for live cell imaging of mitochondria," Bioorg. Med. Chem. Lett. 25 (3), 664-667 (2015).
  • L.-d. Chiu, A. F. Palonpon, N. I. Smith, S. Kawata, M. Sodeoka, K. Fujita, "Dual-polarization Raman spectral imaging to extract overlapping moleclular fingerprints of living cells," J. Biophotonics, 8 (7), 546-6554 (2015).
  • H. Lee, R. Oketani, Y.-T. Huang, K.-Y. Li, Y. Yonemaru, M. Yamanaka, S. Kawata, K. Fujita, S.-W. Chu, "Point spread function analysis with saturable and reverse saturable scattering," Opt. Express, 22 (21), 26016 (2014).
  • N. I. Smith, K. Mochizuki, H. Niioka, S. Ichikawa, N. Pavillon, A. J. Hobro, J. Ando, K. Fujita, Y. Kumagai, "Laser-targeted photofabrication of ​gold nanoparticles inside cells," Nat. Commun., 5, 5144 (2014).
  • K.-C. Huang, K. Bando, J. Ando, N. I. Smith, K. Fujita, S. Kawata, "3D SERS (surface enhanced Raman scattering) imaging of intracellular pathways," Methods, 68 (2), 348 (2014).
  • Y. Yonemaru, M. Yamanaka, N. I. Smith, S. Kawata, K. Fujita, "Saturated excitation microscopy with optimized excitation modulation," ChemPhysChem, 15 (4), 743 (2014).
  • A. Hashimoto, L.-d. Chiu, K. Sawada, T. Ikeuchi, K. Fujita, M. Takedachi, Y. Yamaguchi, S. Kawata, S. Murakami, E. Tamiya, "In situ Raman imaging of osteoblastic mineralization," J. Raman Spectrosc., 45 (2), 157 (2014).
  • S.-W. Chu, T.-Y. Su, R. Oketani, Y.-T. Huang, H.-Y. Wu, Y. Yonemaru, M. Yamanaka, H. Lee, G.-Y. Zhuo, M.-Y. Lee, S. Kawata, and K. Fujita, "Measurement of a saturated emission of optical radiation from gold nanoparticles: application to an ultrahigh resolution microscope," Phys. Rev. Lett., 112 (1), 017402 (2014). (highlighted in APS physics spotlights)
  • T. Ichimura, L.-d. Chiu, K. Fujita, S. Kawata, T. M. Watanabe, T. Yanagida, H. Fujita, "Visualizing cell state transition using Raman spectroscopy," PLOS ONE, 9 (1), e84478 (2014).
  • S.-W. Chu, H.-Y. Wu, Y.-T. Huang, T.-Y. Su, H. Lee, Y. Yonemaru, M. Yamanaka, R. Oketani, S. Kawata. S. Shoji, and K. Fujita, "Saturation and reverse saturation of scattering in a single plasmonic nanoparticle," ACS Photonics, 1 (1), 32-37 (2014). (introduced in Nature Photonics, 8, 92 (2014).
  • H. Yamakoshi, A. F. Palonpon, K. Dodo, J. Ando, S. Kawata, K. Fujita, M. Sodeoka, "Simultaneous imaging of protonated and deprotonated carbonylcyanide p-trifluoromethoxyphenylhydrazone in live cells by Raman microscopy," Chem Commun., 50, 1341 (2014).
  • M. Yamanaka, Y. Yonemaru, S. Kawano, K. Uegaki, N. I. Smith, S. Kawata, K. Fujita, "Saturated excitation microscopy for sub-diffraction-limited imaging of cell clusters," J. Biomed. Opt., 18 (12), 126002 (2013).
  • M. Yamanaka, K. Saito. N. I. Smith, S. Kawata, T. Nagai, K. Fujita, "Saturated excitation (SAX) of fluorescent proteins for sub-diffraction-limited imaging of living cells in three dimensions," Interface FOCUS, 3, 20130007 (2013).
  • A. F. Palonpon, J. Ando, H. Yamakoshi, K. Dodo, M. Sodeoka, S. Kawata, K. Fujita, "Raman and SERS microscopy for molecular imaging of live cells," Nat. Protoc., 8, 677 (2013).
  • T. Shimozawa, K. Yamagata, T. Kondo, S. Hayashi, A. Shitamukai, D. Konno, F. Matsuzaki, J. Takayama, S. Onami, H. Nakayama, Y. Kosugi, T. M. Watanabeg, K. Fujita, Y. Mimori-Kiyosue, "Improving spinning disc confocal microscopy by preventing pinhole cross-talk for intravital imaging," Proc. Nat. Acad. Sci. USA., 110 (9), 3399 (2013).
  • N. Pavillon, K. Bando, K. Fujita, and N. I. Smith, "Feature-based recognition of Surface-enhanced Raman spectra for biological targets," J. Biophoton., 6 (8), 587 (2013).
  • M. Okada, N. I. Smith, A. F. Palonpon, H. Endo, S. Kawata, M. Sodeoka, K. Fujita, "Label-free Raman observation of cytochrome c dynamics during apoptosis," Proc. Natl. Acad. Sci. USA, 109 (1) 28-32 (2012).
  • H. Yamakoshi ,K. Dodo , A. F. Palonpon , J. Ando , K. Fujita , S. Kawata, and M. Sodeoka, "Alkyne-tag Raman imaging for visualization of mobile small molecules in live cells," J. Am. Chem. Soc., 134, 20681-20689 (2012).
  • Y. Masago, A. Hosoya, K. Kawasaki, S. Kawano, A. Nasu, J. Toguchida, K. Fujita, H. Nakamura, G. Kondoh, and K. Nagata, "Molecular chaperone Hsp47 is essential for cartilage and endochondral bone formation," J. Cell Science, 125 (Pt 5), 1118-1128 (2012).
  • J. Ando, K. Fujita, N. I. Smith and S. Kawata, "Dynamic SERS imaging of cellular transport pathways with endocytosed gold nanoparticles," Nano Lett., 11 (12), 5344-5348 (2011).
  • M. Yamanaka, Y-. K. Tzeng, S. Kawano, N. I. Smith, S. Kawata, H-. C. Chang, and K. Fujita, "SAX microscopy with fluorescent nanodiamond probes for high-resolution fluorescence imaging," Biomed. Opt. Express, 2 (7), 1946-1954 (2011)
  • M. Honda, Y. Saito, N. I. Smith, K. Fujita, and S. Kawata, "Nanoscale heating of laser irradiated single gold nanoparticles in liquid," Opt. Express, 19 (13), 12375-12383 (2011).
  • M.-L. Zheng, K. Fujita, W.-Q. Chen, X.-M. Duan, and S. Kawata, "Two-photon excited fluorescence and second-harmonic generation of the DAST organic nanocrystals," J. Phys. Chem. C, 115 (18), 8988-8993 (2011).
  • H. Yamakoshi, K. Dodo, M. Okada, J. Ando, A. Palonpon, K. Fujita, S. Kawata, and M. Sodeoka, "Imaging of EdU, an alkyne-tagged cell proliferation probe, by Raman microscopy," J. Am. Chem. Soc., 133 (16), 6102–6105 (2011).
  • S. Kawano, N. I. Smith, M. Yamanaka, S. Kawata and K. Fujita, "Determination of the expanded optical transfer function in saturated excitation imaging and high harmonic demodulation," Appl. Phys. Express, 4, 042401 (2011).
  • M.-L. Zheng, K. Fujita, W.-Q. Chen, N. I. Smith, X.-M. Duan, and S. Kawata, "Comparison of staining selectivity of subcellular structures by carbazole-based cyanine probes for nonlinear optical microscopy," ChemBioChem., 12, 52-55 (2011).
  • R. J. Milewski, Y. Kumagai, K. Fujita, D. M. Standley, N. I. Smith, "Automated processing of label-free Raman microscope images of macrophage cells with standardized regression for high-throughput analysis," Immunome Research, 6, 11 (2010).
  • M.-L. Zheng, W.-Q. Chen, K. Fujita, X.-M. Duan, and S. Kawata, "Dendrimer adjusted nanocrystals of DAST: organic crystal with enhanced nonlinear optical properties," Nanoscale, 2, 913 - 916 (2010).
  • T. Minamikawa, M. Hashimoto, K. Fujita, S. Kawata, and T. Araki, "Multi-focus excitation coherent anti-Stokes Raman scattering (CARS) microscopy and its applications for real-time imaging," Opt. Express, 17, 9526-9536 (2009). (selected for the publication in Virtual Journal for Biomedical Optics, 4 (8), 2009)
  • K. Fujita, S. Ishitobi, K. Hamada, N. I. Smith, A. Taguchi, Y. Inouye, and S. Kawata, "Time-resolved observation of surface-enhanced Raman scattering from gold nanoparticles during transport through a living cell," J. Biomed. Opt. 14, 024038 (2009).
  • M. Ogawa, Y. Harada, Y. Yamaoka, K. Fujita, H. Yaku, T. Takamatsu, "Label-free biochemical imaging of heart tissue with high-speed spontaneous Raman microscopy," Biochem. Biophys. Res. Communn., 382, 370-374 (2009).
  • J. Ando, N. I. Smith, K. Fujita, and S. Kawata, "Photogeneration of membrane potential hyperpolarization and depolarization in non-excitable cells," Eur. Biophys. J., 38, 255-262 (2009).
  • H. Ishitobi, T. Kai, K. Fujita, Z. Sekkat, and S. Kawata, "On fluorescence blinking of single molecules in polymers," Chem. Phys. Lett. 468, 234-238 (2009).

Review Articles

Invited Talks (International)
  • K. Fujita, "Raman microscopy: a new imaging modality that opens up analytical biology," SCIX2022 (Covington, 5 Oct 2022).
  • K. Fujita, "Detecting drugs in cells and tissues by Raman/SERS microscopy," SCIX2022 (Covington, 4 Oct 2022).
  • K. Fujita, "On-the-fly Raman image microscopy with guaranteeing accuracy using reinforcement learning PART II: Experiment," SCIX2022 (Covington, 4 Oct 2022).
  • K. Fujita, "Label-free imaging of cell spheroids by slit-scanning Raman microscopy," The 15th Asia Pacific Physics Congress (online, 25 Aug 2022).
  • K. Fujita, "Detecting drugs in cells and tissues by Raman/SERS microscopy," 27th International Conference on Raman Spectroscopy (ICORS) (Long Beach, 16 Aug 2022).
  • K. Fujita, "Line-illumination Raman microscopy for imaging biological samples," Conference on Lasers and Electro-optics PacificRim (CLEO-PR) 2022 (Sapporo, 4 Aug 2022).
  • K. Fujita, "Raman microscopy: a new modality to probe cell activity by listening to molecular vibrations," TSRC Workshop (Telluride, 21 Jun 2022).
  • K. Fujita and K. Koike, "Slit-scanning Raman microscopy using Bessel beam illumination" SPIE Photonics West (San Francisco, 23 January 2022).
  • K. Fujita and K. Koike, "Enhancing Raman microscopy by structured light" SPIE Photonics West (San Francisco, 22 January 2022).
  • K. Fujita, "Vibrational-tag SERS microscopy for detecting intracellular molecules and environments," Pacifichem 2021 (online, 16-21 December 2021).
  • K. Fujita, "Intracellular chemical imaging by surface enhanced Raman scattering (SERS) microscopy," International Forum of Microscopy 2021, Guilin (online, 15 Nov 2021). Keynote
  • K. Fujita, "High-speed and High-resolution Raman Imaging of Biological Molecules Using Line Illumination," Asia Communications and Photonics Conference 2021, Shanghai (online, 25 Oct 2021).
  • K. Fujita, " Raman imaging of intracellular structures with improved image contrast," The 11th International Conference on Advanced Vibrational Spectroscopy (ICAVS11) (online, 25 August 2021).
  • K. Fujita, “Photo-initiator free two-photon polymerization using nonlinear deep-UV excitation, SPIE Optics + Photonics 2021 (San Diego (hybrid), 1 August 2021).

    ..... and more

  • H. Yamakoshi, K. Fujita, “Spontaneous Raman and SERS microscopy for Raman tag imaging,” in Stimulate Raman Scattering Microscopy, pp. 275-287 (J.-X. Chen et al Ed, Elsevier 2021).
  • K. Fujita, "Super-Resolution Imaging in Raman Microscopy" in Label-Free Super-Resolution Microscopy, Astratov Ed. (Springer Nature, 2019).
  • K. Mochizuki, N. I. Smith, K. Fujita, "Raman microscopy," Reference Module in Chemistry, Molecular Sciences and Chemical Engineering (Elsevier)
  • J. Ando, K. Dodo, K. Fujita, M. Sodeoka, "Visualizing Bioactive Small Molecules by Alkyne Tagging and Slit-Scanning Raman Microscopy, "Visualizing bioactive small molecules by alkyne tagging and slit-scanning Raman microscopy," Systems Chemical Biology: Methods and Protocols, Methods in Molecular Biology, Vol.1888, pp. 99-114 (Springer Nature 2019).
  • K. Fujita, "Micro-Raman Spectroscopy," in Compendium of Surface and Interface Analysis, pp. 375-379 (Springer Singapore, 2018).
  • K. Fujita, K. Mochizuki, N. I. Smith, "SAX microscopy and its application to imaging of 3D cultured cells," in Super-resolution imaging in biomedicine (A. Diaspro and M. A. M. J. van Zandvoort Eds., CRC Press, October 14, 2016).
  • M. Hashimoto, T. Ichimura, K. Fujita, "CARS Microscopy: Implementation of Nonlinear Vibrational Spectroscopy for Far-Field and Near-Field Imaging," in Raman Imaging, Springer Series in Optical Sciences 168 (A. Zoubir Ed., Springer-Verlag Berlin Heidelberg 2012).
  • Yasuaki Kumamoto, Nicholas Isaac Smith, Katsumasa Fujita, Jun Ando and Satoshi Kawata, "Optical Techniques for Future Pacemaker Technology," in Modern Pacemakers - Present and Future (M. K. Da ed. InTech 2011).
  • N. I. Smith, S. Kawano, M. Yamanaka, and K. Fujita, "Nonlinear Fluorescence Imaging by Saturated Excitation," in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, pp. 2-1 ~ 2-16 (A. Diaspro Ed., Chapman and Hall/CRC press, April 2010).
  • P. Verma, K. Fujita, T. Ichimura, and S. Kawata, "Raman, CARS and near-field Raman-CARS microscopy for cellular and molecular imaging," in "Nano Biophotonics - Science and Technology -," pp. 57-71 (H. Masuhara, S. Kawata, and F. Tokunaga Ed. Elservier B.V., Amsterdam, 2007).
  • K. Fujita, N. Smith, and O. Nakamura, "Nonlinear optical imaging and stimulation of living cells," in Nanophotonics -Intengrating photochemistry, Optics and Nano/Bio Materials Studies - (H. Masuhara and S. Kawata Ed. Elsevier B.V., Amsterdam, 2004).
  • K. Fujita and T. Takamatsu, "Real-time in situ calcium imaging with single and two-photon confocal microscopy," in Confocal and two-photon microscopy: Foundation, Application and Advances (A. Diaspro Ed. John Wiley & Sons, Inc., New York, 2001).

    ..... and more


  • “A further leap of biomedical Raman imaging,” Spectroscopy, Vol. 35, Issue 7, p.10 (2020).
  • “Technology Feature | Live-cell imaging: Deeper, faster, wider,” Science, Vol. 359, Issue 6383, pp. 1549 (2018).
  • "Raman Imaging of Live Cells," Spectroscopy, 18 Nov (2014).
  • "Harmonic Microscopy," RIKEN Research 14 Mar (2008).
  • Richard Gaughan, "Laser Technique Monitors Calcium," PHOTONICS SPECTRA, January, p.65 (2002).
  • Richard Gaughan, "Near-infrared laser generates calcium waves," Biophotonics International, March, p.54 (2002).