FOM2000, SHIRAHAMA, JAPAN, APRIL 9-13, 2000
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FOM2000 CONFERENCE PROGRAM (Provisional)

Revised at 10:30 (local time in Japan) on March 21, 2000
4/9/2000, Sunday
17:00-19:00
REGISTRATION HOUR

19:00-21:00
WELCOME PARTY
4/10/2000, Monday
8:30-9:15
OPENING SESSION

Opening Remark
T. Tsuruta, Vice President, Nikon, Co., JAPAN

Welcome Address (MaAB1)
S. Kawata, Osaka University

9:15
Plenary Talk (MaAB2)
"Intracellular Nanosurgery Using Near Infrared Femtosecond Laser Pulses,"
K. Konig, W. Fritzsche, I. Riemann, U. Tirlapur, K. J. Halbhuber, University Jena, GERMANY

10:00
Plenary Talk (MaAB3)
"Laser Technology for Multiphoton Microscopy,"
K. Ueda, University of Electro-Communications, JAPAN
10:45 - 11:10 (11:20)
COFFEE BREAK
PARALLEL SESSION
ROOM AROOM B
CARS, SHG, and Nonlinearities 1 Calcium Ion Imaging
MaA1(Invited Talk)11:1011:20MaB1
"CARS Microscopy: Selectivity without Staining,"
A. Zumbusch, Universitat Munchen, GERMANY		 "Effect of Low Temperature on Degranulation and Intracellular Ca2+ Changes of Mast CellsA Novel Quantitative Analysis by Real-Time Confocal Microscopy,"
Y. Satoh, S. Mori, T. Saino, T. Akasaka, Iwate Medical University, JAPAN
MaA211:4011:40MaB2
"Molecular Vibrational Imaging in the Fingerprint Region by CARS Microscopy,"
M. Hashimoto, T. Araki, S. Kawata, Osaka University, JAPAN		 "Rapid Scanning Confocal Imaging of Calcium Transients, Waves and Sparks in Atrial and Ventricular Cardiomyocytes,"
H. Tanaka, H. Masumiya, T. Sekine, J. Kase, T. Kawanishi, T. Hayakawa, S. Miyata, Y. Sato, R. Nakamura, K. Shigenobu, Toho University School of Pharmaceutical Sciences, JAPAN
MaA312:0012:00MaB3
"Raman Spectrum Enhancement by Simplex Projection,"
M. Lim and C. Saloma, University of the Philippines, PHILIPPINES		 "Three Types of Ca2+ Waves in the Whole Hearts of a Rat Revealed by Real-Time Confocal Microscopy,"
T. Kaneko, H. Tanaka, M. Oyamada, S. Kawata, T. Takamatsu, Osaka University, JAPAN
MaA412:2012:20MaB4
"Second Harmonic Generation Microscopic Imaging of Teeth,"
F. J. Kao, M. K. Huang, Y. S. Wang, S. L. Huang, P. C. Cheng, National Sun Yat-sen University, TAIWAN		 "The Production of Nitric Oxide Derived by Oxidative Stress on Cardiomyocyte,"
S. Miyata and Y. Satoh, Iwate Medical University, JAPAN
12:40 - 14:20
LUNCH & EXHIBITS
PARALLEL SESSION
ROOM AROOM B
CARS, SHG, and Nonlinearities 2Living Cell Microscopy 1
MpA1 (Invited Talk)14:2014:20MpB1 (Invited Talk)
"Simultaneous Multichannel Nonlinear Imaging,"
R. Gauderon, P. B. Lukins, C. J. R. Sheppard, University of Sydney, AUSTRALIA 		"Induction of Alterations in Mitochondrial Physiology by Oxidant Stress,"
A. Takahashi, University of Texas Health Scienec Center at San Antonio, USA
MpA214:5014:50MpB2
"Two-Photon Fluorescence Energy Transfer,"
M. G. Xu, S. M. Russell, B. Crimeen, M. J. Ludford-Menting, X. Gan, M. Gu, Swinburne University of Technology, AUSTRALIA		 "Two-Photon Micro-Spectroscopy of Chloroplasts from Arabidopsis Thaliana,"
F. J. Kao, B. L. Lin, P. C. Cheng, National Sun Yat-sen, University, TAIWAN
MpA315:1015:10MpB3
"Mapping Piezoelectric Field Distribution in Gallium Nitride by Scanning Second-Harmonic Generation Microscopy,"
C. K. Sun, S. W. Chu, S. P. Tai, National Taiwan University, TAIWAN R.O.C 		"The Differential Structure of an Image as a Tool for the Detection of Geometrical Features in Curvilinear Structures,"
G. J. Streekstra, R. van den Boomgaard, A. W. M. Smeulders, G. J. Brakenhoff, University of Amsterdam, THE NETHERLANDS
Theory and Instrumentation 1
MpA4 (Invited Talk)15:3015:30MpB4
"Wide-Field Fluorescence Microscopy with 100 nm XYZ Resolution,"
M. G. L. Gustafsson, University of California, USA		 "Syndecan-1 (CD 138) Reactivity in Dividing Cells,"
U. Brockstedt, B. Axelsson, K. Dobra, A. Hjerpe, Huddinge University of Hospital, SWEDEN
MpA516:0015:50MpB5
"Confocal Fluorescence Microscopy Using CCD-Sensors _ Speed and Low Cost through More Electronics,"
R. E. Grofikopf, ConfImagingResarch (CIR), GERMANY		 "Calcium-Independent Irreversible Perturbation of Regulatory Mechanisms of Intra-cellular Ion Concentration Induced by Glutamate in Rat Hippocampal Neuronal Cells,"
T. Kawanishi, M. Yamamoto, S. Yamakawa, N. Takada, R. Shibayama, H. Ohata, K. Momose, T. Hayakawa, National Institute of Health Sciences, JAPAN
16:20 (16:10) - 16:40
COFFEE BREAK
PARALLEL SESSION
ROOM AROOM B
Theory and Instrumentation 2Biophysics & Cell Damage
MpA6 (Invited Talk)16:4016:40MpB6 (Invited Talk)
"Wavefront Control in Confocal Microscopy,"
T. Wilson, University of Oxford, UK		 "Topologocal Transformations of Lipid Bilayer Vesicles,"
F. Nomura, M. Honda, S. Takeda, K. Takiguchi, H. Hotani, Nagoya University, JAPAN
MpA717:1017:10MpB7
"Extended-Depth-of-Focus Fluorescence Microscope Made Possible Using Wavefront Coding,"
C. J. Cogswell, M. R. Arnison, E. R. Dowski, S. C. Tucker, W. T. Cathey, University of Sydney, AUSTRALIA		 "Single Molecule Signal Fluctuations Analyzed via CLSM-Integrated Confocal Fluorescence Correlation Spectroscopy (CFCS),"
N. Opitz, A. Kramer, J. Kuhlmann, A. Wittinghofer, Max-Planck-Institute for Molecular Physiology, GERMANY
MpA817:3017:30MpB8
"Adapative Optics in Microscopy,"
M. A. Neil, M. J. Booth, T. Wilson, Oxford University, UK		 "Observation of the Diffusion of Single Dye Labelled Viruses in Live Cells,"
G. Seisenberger, M. Ried, M. Hallek, C. Brauchle, A. Zumbusch, Universitat Munchen, GERMANY
MpA917:5017:50MpB9
"Automated Tracking of the Spherical Aberration Induced by Complex Specimen,"
F. Margadant, University of Sydney, AUSTRALIA		 "The Response of Maize Protoplasts to High Intensity Illumination in Multi-photon Fluorescence Microscopy,"
P. C. Cheng, F. J. Kao, B. L. Lin, W. Y. Cheng, State University of New York, USA
MpA1018:1018:10MpB10
"Three-dimensional Microscopy using Polarisation Patterns,"
R. Juskaitis, M. A. A. Neil, L. Sucharov, and T. Wilson, Oxford University, UK		 "Estimation of Burn Depth by Fibre Optic Confocal Microscopy (FOCI) _ Evidence that Cool Water Treatment Limits Progressive Thermal Damage,"
L. T. VO, P. Anikijenko, P. M. Delaney, R. G. King, Monash University, AUSTRALIA
MpA1118:30
"Backscattered Light: The Forgotten Signal in Biolog, "
J. Pawley, F. Margadant, University of Sydney, AUSTRALIA
19:00-21:00
CARL ZEIZZ SEMINAR
NLO510 and Fluorescence Correlation Microscopy
(Complimentary snacks and beverages will be available)
4/11/2000, Tuesday
8:30
Plenary Talk (TaAB1)
"Non Linear Optical Microscopy,"
G. J. Brakenhoff, J. Squier, University of Amsterdam, THE NETHERLANDS

9:15
Pleanary Talk (TaAB2)
"3D-Microscopy in Pathology from Cell to Organ,"
T. Takamatsu, Kyoto Prefectural University of Medicine, JAPAN
10:00 - 10:30
COFFEE BREAK
PARALLEL SESSION
ROOM AROOM B
Laser TrappingLiving Cell Microscopy 2
TaA1 (Invited Talk)10:3010:30TaB1 (Invited Talk)
"Scattering and Depolarization in Particle-Trapped Near-Field Scanning Microscopy,"
M. Gu, Swinburne University of Technology, AUSTRALIA		 "Real time confocal imaging in living cells: from milliseconds to hours,"
Peter Lipp, Babraham Institute, Biology Biotechnology Science Council, UK
TaA211:0011:00TaB2
"Surface-Force Measurement Using a Laser-Trapped Particle Probe,"
T. Ohta, T. Sugiura, and S. Kawata, Osaka University, JAPAN		 "Three-Dimensional Subcellular Observation of Cardiac Myocytes during Looping of Whole-Mounted Chiken Embryo,"
I. Shiraishi, M. Oyamada, K. Hamaoka, T. Takamatsu, Kyoto Prefectural University of Medicine, JAPAN
TaA311:2011:20TaB3
"Photonic Force Microscopy,"
E. Florin, J. K. H. Horber, E. H. K. Stelzer, European Molecular Biology Laboratory(EMBL), GERMANY		 "High Resolution and High Speed Dual-Color Imaging of Spatial Cellular Structures in Live Cells,"
R. Elis, D. Gerlich, A. Rustom, M. Gebhard, R. Rudolf, W. Tvarusko, H. H. Gerdes, German Cancer Research Center, GERMANY
TaA411:4011:40TaB4
"Trapping and Tracking of Dielectric Particles in the Presence of External Disturbances,"
A. Rohrbach, E. H. K. Stelzer, European Molecular Biology Laboratory (EMBL), GERMANY 		"4D-Imaging of Living Cell,"
E. Manders, A. E. Visser, A. Koppen, R. van Driel, G. J. Brakenhoff, University of Amsterdam, THE NETHERLANDS
Green Fluorescent Protein
TaA512:0012:00TaB5
"Comparison of Microobjects Optical Trapping by Single Beam and Standing Wave,"
A. Jonas, J. Jezek, M. Sery, P. Jakl, P. Zemanek, M. Liska, Academy of Science of the Czech Republic, CZECH REPUBLIC		 "Nuclear Translocation of NF-B with GFP in PC12 Cells,"
T. Furuno, N. Hirashima, M. Nishio, M. Nakanishi, Nagoya City University, JAPAN
TaA612:2012:20TaB6
"Application of Laser-Trapping Technique for Measuring of Viscosity Distribution in Three Dimensions,"
B. Kolodziejczyk, R. Lugowski, Y. Kawata, Shizuoka University, JAPAN		 "Photodynamics of the GFP Probed with Fluorescence Correlation Spectroscopy (FCS),"
G. Jung, J. Wiehler, B. Steipe, C. Brauchle, A. Zumbusch, Universitat Munchen, GERMANY
12:40 - 14:20
LUNCH & EXHIBITS
PARALLEL SESSION
ROOM AROOM B
High resolution Microscopy and Instrumentation X-Ray Microsocopy
TpA1 (Invited Talk)14:2014:20TpB1 (Invited Talk)
"Confocal Microscopy Sharpened Up,"
S. W. Hell, T. A. Klar, M. Dyba, S. Jakobs, MPI for Biophysical Chemistry, GERMANY 		"X-ray Cone-beam Microtomography Using a Cylindrical Coordinate System,"
P. C. Cheng, State University of New York, USA
TpA214:5014:50TpB2
"Non-Linear Microscopy without Non-Linear Susceptibilities and Far-Field Microscopy Concepts with Resolution at Molecular Scale,"
S. W. Hell, A. Schonle, MPI for Biophysical Chemistry, GERMANY		 "X-ray Refraction Imaging Using Microfocus X-Ray Tube,"
M. Ukita, M. Takebe, S. Hayashi, T. Yoshida, Shimadzu Corporation, JAPAN
TpA315:1015:10TpB3
"High Resolution Confocal Microscope Featuring Semiconductor Blue Laser Diode,"
T. Ode, Lasertech, JAPAN		 "HgBrI New Semiconductor Material for X and flRay Investigated by Using aConfocal Laser Scanning Microscope,"
G. A. Stanciu, M. H. Oprica, University Politehnica of Bucharest, ROMANIA
Data Processing
TpA415:3015:30TpB4
"Imaging with Berry Phase in Polarization Interferometer with Geometric Shear,"
A. V. Tavrov, Y. Miyamoto, T. Kawabata, M. Takeda, V. A. Andreev, The University of Electro-Communications, JAPAN		 "Dynamic Data ExtractionA Java Application for Confocal Microscope Image Files,"
M. Esterman, J. R. Delbauve, Lilly Research Laboratories Information Technology, USA
TpA515:5015:50TpB5
"Three Dimensional Surface Topography Characterization of Engineered Surfaces,"
U. Wendt, M. Thiel, S. Traufelder, Otto-von-Guericke-University, GERMANY 		"Quantification of Errors Made in Deconvolution under the Assumption of PSF Depth Invariance,"
C. Xu, O. Haeberle, B. Colicchio, S. Razgallah, Universite de Haute-Alsace, FRANCE
TpA616:1016:10TpB6
"Developments in 4Pi-Confocal Microscopy,"
M. Nagorni, K. Bahlmann, H. Kano, S. W. Hell, MPI for Biophysical Chemistry, GERMANY 		"Analysis of Fast Dynamic Processes in Living CellsHigh Resolution and High Speed Dual-Color Imaging Combined with Automated Image Analysis,"
H. H. Gerdes, A. Rustom, D. Gerlich, R. Rudolf, C. Heinemann, R. Eils, University of Heidelberg, GERMANY
16:30-18:00
POSTER SESSION & EXHIBITS (coffee available)
TpP1TpP2
"New Imaging Modes for Lenslet-Array TSMs,"
T. F. Watson, R. Juskaitis, T. Wilson, Oxford University, UK		 "Transient Response of Neurotrophins on Intracellular Ca Concentration and Synaptic Activity in Cultured Rat Cortex,"
K. Torimitsu, Y. Furukawa, N. Kasai, Y. Jimbo, NTT Basic Research Laboratories, JAPAN
TpP3TpP4
"Effect of the Detector Side Pinhole on 3D Optical Properties of CARS Microscopy,"
K. Inoue, M. Hashimoto, T. Araki, Osaka University, JAPAN		 "Realization and Characterization of a National TPE Architecture Based on a Compacts Scanning Head,"
A. Diaspro, M. Robello, University of Genoa, ITALY
TpP5TpP6
"Confocal Polarization Microscopy for the Analysis of Fluorescent Materials,"
W. Inami, Y. Kawata, Shizuoka University, JAPAN		 "A Magneto-Optic Scanning Laser Microscope Facility for the Time-ResolvedExamination of Domain Dynamics,"
W. Clegg, L. He, D. Jenkins, J. Windmill, University of Plymouth, UK
TpP7TpP8
"Three-Dimensional Theory of Phase Imaging in a Holographic Confocal Microscopy,"
R. Chmelik, Z. Harna, P. Knoll, Brno University of Technology, CZECH REPUBLIC 		"A Two-photon Multifocus Confocal Microscopy for Real-time Cell Imaging,"
K. Fujita, T. Kaneko, O. Nakamura, M. Oyamada, T. Takamatsu, S. Kawata, Osaka University, JAPAN
TpP9TpP10
"Imaging Properties of Laser Scanning Differential Phase Contrast Microscopy with Four Split Photo Detector,"
T. Tanaka, T. Kawano, S. Yamamoto, Osaka University, JAPAN		 "An Application of Surface Roughness with CLSMAn Algorithm to Enhance the Depth Discrimination Properties,"
A. Baldi, F. Ginesu, M.Lera, C.J.R. Sheppard, Universita Di Cagliari, ITALY
TpP11TpP12
"Evaluation of Fluorochromes for 2-Photon Laser Scanning Microscopy (2-PLSM) of Environmental Biofilm Systems,"
T. R. Neu, J. R. Lawrence, UFZ Center for Environmental Research, GERMANY 		"Tomographic Microscope with Superimposition of Fluorescence and Interference Contrast,"
T. V. Bulygin, G. G. Levin, G. N. Vishnyakov, All-Rusian. Rsch Institute for Optophysical. Measurements, RUSSIA
TpP13TpP14
"Three Dimensional Imaging of Tumor Angiogenesis in Human Breast Cancer by Confocal Laser Scanning Microscopy (CLSM),"
J. Itoh, K. Kawai, A. Serizawa, K. Ogawa, K. Yasumura, R. Y. Osamura, Tokai University, JAPAN 		"Real-Time Three-Dimensional Imaging of Mechanical Stress-Induced Localized Ca2+ Influx by using Multi-Pinhole Confocal Disk Scanner,"
H. Ohata, M. Yamamoto, N. Maeyama, T. Ikeuchi, T. Miyazaki, K. Momose, Showa University, JAPAN
TpP15TpP16
"Ultrasound-Assisted Optical Measurement for Thick Tissue Samples ,"
M. Hisaka, T. Sugiura, S. Kawata, Osaka University, JAPAN 		"Three-Dimensional Fabrication and Three-Dimensional Observation of Micro-Structures Using Two-photon Absorption ,"
T. Tanaka, S. Kawata, Osaka University, JAPAN
TpP17
"Photofabrication of Three-Dimensional Photonic Crystal by Multi-Beam Interference into Photopolymerizable Resin ,"
S. Shoji, S. Kawata, Osaka University, JAPAN
4/12/2000, Wednesday
PARALLEL SESSION
ROOM AROOM B
Near Field Scanning Microscopy Contrast Mechanism
WaA1 (Invited Talk)8:308:30WaB1 (Invited Talk)
"Nanostructures in Luminescent Conjugated Polymers Thin Films Studied by Polarization Modulation Near-Field and Confocal Optical Microscopy,"
W. S. Fann, National Taiwan University, TAIWAN		 "Using Heisenberg's Uncertainty Principle to Estimate Focal SpotDimensionnns,"
E. H. K. Stelzer, S. Grill, European Molecular Biology Laboratory(EMBL), GERMANY
WaA29:009:00WaB2
"Near-Field Surface Enhanced Raman Spectroscop,"
N. Hayazawa, Y. Inouye, Z. Sekkat, S. Kawata, Osaka University, JAPAN		 "Imaging of Anisotropic Point Objects by Polarized Light Scanning Optical Microscopes,"
P. Torok, University of Oxford, UK
Tissue & Skin Imaging
WaA39:209:20WaB3
"Near Field Optical Image of Refractive Objects; Numerical Analysis of the Border Contrast,"
M. Hattori, S. Komatsu, Waseda University, JAPAN		 "Diffraction Effects in Focused Pulsed Light Propagation through Highly-Scattering MediaModified Monte Carlo Modeling,"
V. Daria, C. Saloma, S. Kawata, University of the Phillipines, PHILIPPINES
WaA4 (Invited Talk)9:409:40WaB4
"Scanning Tunnelling Spectroscopic Imaging of Light-Sensitive Biological Molecules,"
P. B. Lukins, University of Sydney, AUSTRALIA		 "Diffraction Effects in Light Propagation through a Highly Scattering Medium,"
C. M. Y. Blanca, J. Bantang, C. Saloma, University of the Philippines, PHILIPPINES
WaA510:1010:00WaB5
"Applications of Scanning Near-Field Optical Microscopy,"
M. Walter, R. Pepperkok, M. Detje OMICRON Vakuumphisk GmbH, GERMANY		 "Strategies for the confocal imaging of skin,"
M. J. Booth and T. Wilson, Oxford University, UK
WaA610:3010:20WaB6
"Near-field Microscopy by Using a Laser-trapped Particle as a Probe,"
T. Sugiura, Osaka University, JAPAN		 "Image Reconstruction through a Tissue-Like Turbid Medium under Single-Photon and Two-Photon Excitation,"
M. Gu, X. Gan, Swinburne University of Technology, AUSTRALIA
10:50 (10:40) - 11:10
COFFEE BREAK

11:10
Plenary Talk (WaAB1)
"Near- and Far Field Measurements at Nanometer Scale,"
L. Aigouy, A. Dubois, A. C. Boccara, Ecole Superieure de Physique et Chimie Industrielles, FRANCE

11:55
Closing Remark
G. J. Brakenhoff, University of Amsterdam, THE NETHERLANDS

14:30 - 17:30
EXCURSION
18:00 - 20:30
CONFERENCE BANQUET
[Home] [Foreword] [Overview] [Program] [Presentation] [Optional_Tour] [Abstract] [Registration] [Accommodation] [Travel_Info.] [Shirahama] [Exhibit] [Organizing_Comittee] [Workshop]